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Flow Cytometry

The Principle of Flow CytometryFlow cytometry (FCM) is a technology developed in the 1970s to quickly and accurately identify and classify immune cells and other cells. It mainly includes fluid flow technology, cell sorting and counting technology, as well as data collection and analysis technology, etc. At present, FCM has become an important biological tool for detecting various components of cells. It can not only quantitatively detect various cellular components in cell membranes, cytoplasm, nucleus, and various soluble biomolecular components in serum, but also study various functional states of cells. In recent decades, with the continuous discovery of new fluorescent substances and the increasing improvement of computer functions in data storage, display analysis, etc., FCM has transitioned from the previous qualitative description to quantitative research, and can conduct data statistics, which has greatly improved the scientific nature of experimental results. Based on the above characteristics, FCM has become an important research technique for clinical diagnosis and has a wide application prospect.

The Workflow of Flow Cytometry

The workflow of flow cytometry is as follows. Planning the workflow in advance as shown in the figure helps to improve the success rate of the experiment.

The Workflow of Flow Cytometry – Creative Biogene

Applications of Flow Cytometry

Flow cytometry is widely used in basic research and clinical practice, and plays an important role in the fields of cell biology, hematology, immunology, oncology, pharmacology, genetics, and clinical testing. It can assist in the diagnosis of various diseases such as blood diseases, cancer, autoimmune diseases, antiviral infections and so on. One of the most common applications is the diagnosis of blood-related cancers, such as leukemia and lymphoma. Take leukemia as an example, use fluorescent dye-treated specific monoclonal antibodies, such as CD2, CD7, CD10, CD14, CD19, CD33, CD34, CD41, CD64 and HLA-DR, etc., to detect the presence or absence of various cell components commonly seen in leukemia, thus achieving the purpose of diagnosis. In the early stage of malignant disease, there may be only a few cancer cells in the sample, and routine examination under the microscope may not be able to find these cancer cells, so flow cytometry is particularly useful in the early stage of malignant disease.

Advantages of Flow Cytometry

Flow cytometry is a new technology that can quickly, accurately, and high-throughput the characteristics or functions of a single particle to perform multi-parameter qualitative and quantitative analysis. The advantage of FCM is that it can quickly measure multiple indicators at the same time, and it transitions the diagnosis result from the previous qualitative description to the quantitative research, which greatly improves the scientific nature of the results.

Creative Biogene has accumulated years of experience in flow cytometry. We promise to provide you with the best diagnostic products. You can choose us with confidence.

Please contact us for more details.

Reference

  1. Peters J M., and Ansari M Q. (2011). "Multiparameter flow cytometry in the diagnosis and management of acute leukemia." Archives of pathology & laboratory medicine. 135.1: 44-54.
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Cat# Product Name Product Type Inquiry
C-FC-0861A Biotin Anti-Human CD235 Antibody FCM Antibody Inquiry
C-FC-0862A Biotin Anti-Human CD274/PD-L1 Antibody FCM Antibody Inquiry
C-FC-0863A Biotin Anti-Human CD29 Antibody FCM Antibody Inquiry
C-FC-0864A Biotin Anti-Human CD3 Antibody FCM Antibody Inquiry
C-FC-0865A Biotin Anti-Human CD3 Antibody FCM Antibody Inquiry
C-FC-0866A Biotin Anti-Human CD32 Antibody FCM Antibody Inquiry
C-FC-0867A Biotin Anti-Human CD35 Antibody FCM Antibody Inquiry
C-FC-0868A Biotin Anti-Human CD38 Antibody FCM Antibody Inquiry
C-FC-0869A Biotin Anti-Human CD4 Antibody FCM Antibody Inquiry
C-FC-0870A Biotin Anti-Human CD41 Antibody FCM Antibody Inquiry
C-FC-0871A Biotin Anti-Human CD45RA Antibody FCM Antibody Inquiry
C-FC-0872A Biotin Anti-Human CD47 Antibody FCM Antibody Inquiry
C-FC-0873A Biotin Anti-Human CD5 Antibody FCM Antibody Inquiry
C-FC-0874A Biotin Anti-Human CD57 Antibody FCM Antibody Inquiry
C-FC-0875A Biotin Anti-Human CD58 Antibody FCM Antibody Inquiry
C-FC-0876A Biotin Anti-Human CD62L Antibody FCM Antibody Inquiry
C-FC-0877A Biotin Anti-Human CD64 Antibody FCM Antibody Inquiry
C-FC-0878A Biotin Anti-Human CD74 Antibody FCM Antibody Inquiry
C-FC-0879A Biotin Anti-Human CD83 Antibody FCM Antibody Inquiry
C-FC-0880A Biotin Anti-Human CD86 Antibody FCM Antibody Inquiry
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