Human Parainfluenza Virus Infection

Human parainfluenza virus (HPIV) can mainly cause lower respiratory tract infections in children, and its pathogenicity is second only to the respiratory syncytial virus (RSV). According to statistics, HPIV can lead to 30% to 40% of acute respiratory infections in infants and young children. HPIV can also cause severe and recurrent pneumonia, bronchitis and bronchiolitis lower respiratory tract diseases, which mainly occur in the elderly and people with immunodeficiency. The disease is prone to repeated infections due to low immunity after infection. There are four types of HPIV, most significant infections are caused by HPIV-1 and HPIV-3 whereas HPIV-4 is the least common cause of disease.

Diagnosis of Human Parainfluenza Virus Infection

The diagnosis of HPIV infection is mainly through the following two methods:

• Pathogen diagnosis. Isolate and identify viruses in cells or directly detect viruses in respiratory secretions, and detect virus-specific antigens in exfoliated epithelial cells with fluorescent antibodies of type 1 to 3 parainfluenza viruses. Besides, the nucleic acid of each type of parainfluenza virus needs to be tested. The test methods include immunofluorescence, PCR, enzyme-linked immunoassay, etc.
• Serological examination. Neutralization tests, complement fixation tests and hemagglutination inhibition tests are applied to detect serum-specific IgG antibodies. The double serum antibody titer in the acute and convalescent phases has increased by more than four times, which is of diagnostic value. The use of enzyme-linked immunosorbent assay to detect acute-phase serum-specific IgM antibodies is helpful for early diagnosis. However, each reinfection can lead to an increase in antibody recall of other types of viruses, so the results of a single serologic test can only be used as a reference.

Advantages of Our Products

Creative Biogene's parainfluenza virus detection is a multiplex real-time PCR, or real-time fluorescent PCR test kit for qualitative detection and identification of viral nucleic acids, which are extracted from nasal swabs, throat swabs and nasopharyngeal swabs of symptomatic patients. This test method has been shown to detect and distinguish between type 1, type 2 and type 3 parainfluenza viruses. Our products have high specificity, no cross-reactivity with influenza A virus, influenza B virus, adenovirus, human Boca virus, EB virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, etc., and the sensitivity and specificity are also good.

Creative Biogene focuses on the field of diagnostic reagents for HPIV infection. We are committed to providing the best products to accelerate the realization of customers' research goals. You can choose us without hesitation.

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Reference

1. Pecchini R, et al. (2015). "Parainfluenza virus as a cause of acute respiratory infection in hospitalized children." The Brazilian Journal of Infectious Diseases. 19. 4:358-362.