Norovirus Infection

In 1968, scientists isolated a viral pathogen from the feces of a patient with acute diarrhea that broke out in Norwalk City, USA, and named it Norwalk virus, also known as Norovirus. It is a group of virus particles with similar morphology and slightly different antigenicity. Norovirus infection affects the stomach and intestines and causes gastroenteritis. The main symptoms of the disease are diarrhea and vomiting. Norovirus is divided into 5 genomes (GⅠ-GⅤ), of which only GⅠ, GⅡ and GⅣ can infect humans, while GⅢ and GⅤ can infect cows and rats respectively. Norovirus infections are prevalent all over the world, and mainly affects adults and school-age children, with high incidence during the cold season. Norovirus is responsible for 60%-90% of all nonbacterial diarrhea outbreaks in the United States each year.

Diagnosis of Norovirus Infection

The diagnosis methods of norovirus infection are as follows:

• Electron microscopy. To confirm the norovirus by observing the morphology of virus particles by the electron microscope, the virus concentration required is relatively high, so the sensitivity and specificity are relatively low, and it can only be carried out in more professional testing institutions.
• Antigen detection. ELISA and other immune methods are used to detect norovirus antigens. However, due to the high variation of norovirus antigens, samples with negative ELISA test results need to be confirmed by a second test using real-time RT-PCR. This method can only be used as an auxiliary detection method.
• Molecular diagnosis. The Real-time is currently the most widely used method for detecting norovirus, and is known as the gold standard for detecting norovirus. The ORF1/ORF2 region is the most conserved region in the genome of norovirus. Different strains of the same genotype have the same conserved sequence. According to this conserved region, it can be used to design primers and primers and probes for TaqMan-based real-time RT-PCR. This method can detect the GⅠ group and GⅡ group of norovirus.

Advantages of Our Products

Creative Biogene's test kits use real-time fluorescent PCR technology, take the highly conserved regions of norovirus GⅠ and GⅡ genomes as the target regions, designs specific primers and fluorescent probes. Then the target sequence is amplified by PCR, and the accumulation of amplified products is monitored by fluorescence probe technology to realize the qualitative detection of the nucleic acid of norovirus in the samples. Our products have high specificity and the test results are accurate and reliable.

Creative Biogene has accumulated years of experience in norovirus infection diagnosis. We promise to provide you with the best diagnostic products. You can choose us with confidence.

Please contact us for more details.

Reference

1. Zhou D M, et al. (2013). "Establish A Duplex Fluorescent Quantitative One-step RT-PCR System for the Detection of Norovirus Genogroup Ⅰ and Ⅱ." Chinese Journal of Virology. 03.