Toxoplasma IgA ELISA

The Toxoplasma IgA ELISA kit is intended for the detection of IgA antibody to Toxoplasma in human serum or plasma. Diluted serum is added to wells coated with purified Toxoplasma antigen. Toxoplasma IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the unknown.