Sm-Ab ELISA

Enzyme immunoassay qualitatively and quantitatively determine the anti-Sm IgG antibody in human serum. The uracil-rich small nuclear RNA (U-snRNA) and protein are physiologically compounded into ribonucleoprotein particles (U-snRNP), and ribonucleoprotein particles are involved in the splicing process of pre-mRNA. Different complexes (U1-U6) can be distinguished according to the RNA involved. Their protein components are partly the same and partly different. Sm autoantibodies (according to the patient Smith described in the first description) target those proteins common to all RNPs. The largest are B, B'and D (28, 29, 16 kDa). These antibodies are highly specific for systemic lupus erythematosus (SLE), but only show limited sensitivity (<30%).