Scl-70-Ab ELISA

Enzyme immunoassay qualitatively and quantitatively determine the anti-Scl-70 IgG antibody (DNA topoisomerase I) in human serum. Progressive systemic sclerosis (PSS, scleroderma) is a chronic inflammation of connective tissue, autoimmune-mediated disease. Its main manifestation is thickening and hardening of the skin, but internal organs are also affected. Autoantibodies against ribozyme DNA topoisomerase I (= Scl-70 antigen) are considered to be reliable markers of PSS (specificity> 90%). They indicate a severe course (diffuse scleroderma), usually involving the lungs, kidneys or heart. In contrast, patients with CREST syndrome (the skin form of PSS is limited) rarely carry Scl-70 autoantibodies.