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| Catalog: | C-EL-2178T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Rat |
| Detection range: | 62.5-4000 pg/mL |
| Sensitivity: | 14.9 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | matrix metallopeptidase 9, Mmp9, MMP-9 |
| Application: | TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-beta appears to promote late stage progression and metastasis in some cancers. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Rat total MMP9 ELISA kit is to be used to detect and quantify protein levels of endogenous rat total MMP9. The assay recognizes rat total MMP9. An antibody specific for rat total MMP9 has been pre-coated onto the microwells. The rat total MMP9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat total MMP9 is added to detect the captured rat total MMP9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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