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| Catalog: | C-EL-2170T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Rat |
| Detection range: | 62.5-4000 pg/ml |
| Sensitivity: | 2.1 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Il10, IL-10, IL10X, interleukin 10 |
| Application: | Fatty acid‐binding protein 1 (FABP1, also known as L‐FABP) is a 14 KDa cytosolic protein abundantly expressed in the liver. It can bind fatty acids and many other molecules and is involved in the uptake, transport, and metabolism of cellular long-chain fatty acids and other lipid ligands. Higher FABP1 serum level has been associated with liver injury. Levels of FABP1 can be helpful as a biomarker for metabolic disturbances and impaired function of hepatocytes. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL10 ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL10. The assay recognizes rat IL10. An antibody specific for rat IL10 has been pre-coated onto the microwells. The rat IL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat IL10 is added to detect the captured rat IL10 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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