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Purified Anti-Mouse CD29 Antibody

  • Product Information
  • Description
Catalog: C-FC-2742A
Product Type: FCM Antibody
Size: 50 μg/100 μg
Reactivity: Mouse
Analysis mode: FCM,WB,IHC,IP
Host: Rat
Isotype: Rat IgG2a, κ
Alternate names: Integrin beta-1,ITB1,Itgb1,Fibronectin receptor subunit beta,VLA-4 subunit beta,CD29
Form: Liquid
Shipping: Biological ice pack at 4℃
Storage: 2-8℃
Conjugation: Unconjugated
Buffer: PBS with 0.05% Proclin300
Swiss port: P09055
Clone NO.: KMI6
Recommended use: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1 μg per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.

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