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PE/Cyanine5 Anti-Mouse CD162 Antibody

  • Product Information
  • Description
Catalog: C-FC-1774A
Product Type: FCM Antibody
Size: 20 tests/50 tests/100 tests/200 tests
Reactivity: Mouse
Analysis mode: FCM
Host: Rat
Isotype: Rat IgG1, κ
Alternate names: P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Form: Liquid
Shipping: Biological ice pack at 4℃
Storage: 2-8℃
Conjugation: PE/Cyanine5
Buffer: PBS with 0.05% Proclin300, 1% BSA
Swiss port: Q62170
Clone NO.: 4RA10
Recommended use: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

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