MPO-Ab (p-ANCA) ELISA

Enzyme immunoassay qualitatively and quantitatively determine IgG antibodies against myeloperoxidase (MPO) in human serum. The anti-neutrophil cytoplasmic antibody (ANCA), originally identified by immunofluorescence assay (IFA), targets the cytoplasmic components of neutrophil granulocytes and monocytes. They have been shown to be useful serological markers for many systemic, autoimmune-mediated vasculitis. The perinuclear (p-ANCA) staining pattern of neutrophil substrates indicates autoantibodies against myeloperoxidase (MPO), which is a 145 kDa cation covalently linked α2-β2 complex Things. MPO autoantibodies are closely related to one or another form of microscopic polyvasculitis. They also occur in patients with multiple vasculitis overlap syndrome without renal involvement, idiopathic or vasculitis-related crescent glomerulonephritis.