Mouse NGAL ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous mouse NGAL. The assay recognizes mouse NGAL. An antibody specific for mouse NGAL has been pre-coated onto the microwells. The mouse NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse NGAL is added to detect the captured mouse NGAL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.