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| Catalog: | C-EL-2143T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 7.8-500 pg/mL |
| Sensitivity: | 1.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | B-cell growth factor II, BCGF-II, Il 5, Il5, IL-5, interleukin 5, Interleukin-5 |
| Application: | IL27, a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties that can enhance or limit immune responses. IL27 is a heterodimeric cytokine composed of two subunits: IL27a and IL27b. IL27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL10 producing type 1 regulatory T cells. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes mouse IL5. An antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL5 is added to detect the captured IL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
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