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| Catalog: | C-EL-2140T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 2.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | IL 27, IL 27p28, Il27, IL-27, Il30, interleukin 27, Interleukin-27, p28 |
| Application: | Interleukin-2 (IL2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL2 signals through heterodimerization of the IL2Rβ and Il-2Rγ receptor subunits. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL27. The assay recognizes mouse IL27. An antibody specific for mouse IL27 has been pre-coated onto the microwells. The mouse IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL27 is added to detect the captured mouse IL27 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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