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| Catalog: | C-EL-2139T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 31.25-2000 pg/mL |
| Sensitivity: | 10.2 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | IL 22, IL TIF, Il22, IL-22, Iltif, interleukin 22 |
| Application: | IL-1Ra, also known as IL-1F3, is a member of the interleukin 1 cytokine family. It is a receptor antagonist of IL-1, can competitively bind with IL-1R1 thereby blocking cell activation by the cytokine. IL-1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. As a potent immune/inflammatory molecule, IL-1Ra is tightly associated with osteomyelitis, rheumatoid arthritis, traumatic brain injury (TBI) and stroke. Recently, an increasing number of studies proved that IL-1Ra is also actively involved in tumor progression, such as high expression IL-1Ra increased the risk of bladder cancer and non-cardia gastric carcinoma. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL22 ELISA kit is to be used to detect and quantify protein levels of endogenous IL22. The assay recognizes mouse IL22. An antibody specific for IL22 has been pre-coated onto the microwells. The IL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL22 is added to detect the captured mouse IL22 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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