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| Catalog: | C-EL-2132T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 2.2 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Il 12a, IL 12p35, IL-12, IL12 p70, Il12a, IL12p70, IL12-p70, interleukin 12a, Interleukin-12, Ll12a, p35 |
| Application: | Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body’s response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer’s disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70. The assay recognizes mouse IL12 p70. An antibody specific for mouse IL12 p70 has been pre-coated onto the microwells. The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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