Mouse Endoglin/CD105 ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Endoglin/CD105 ELISA kit is to be used to detect and quantify protein levels of endogenous Endoglin/CD105. The assay recognizes mouse Endoglin/CD105. An antibody specific for Endoglin/CD105 has been pre-coated onto the microwells. The Endoglin/CD105 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Endoglin/CD105 is added to detect the captured Endoglin/CD105 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.