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| Catalog: | C-EL-2117T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 5.7 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | C-C motif chemokine 5, Ccl5, chemokine (C C motif) ligand 5, MuRantes, RANTES, Scya5, SISd, Small-inducible cytokine A5, TCP228 |
| Application: | The C-C motif chemokine ligand 22 (CCL22), also known as MDC, belongs to the group of chemokines, that are both constitutively expressed under homeostatic conditions and inducible upon inflammation. CCL22 is a secreted protein that exerts chemotactic activity for monocytes, dendritic cells, natural killer cells, and for chronically activated T lymphocytes. CCL22 is induced by LPS, IL-4, and IL-13 and in T cells by TCR stimulation. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL5 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL5. The assay recognizes mouse CCL5. An antibody specific for mouse CCL5 has been pre-coated onto the microwells. The mouse CCL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL5 is added to detect the captured mouse CCL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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