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| Catalog: | C-EL-2116T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 5.0 pg/mL |
| Sample type: | Cell culture supernatant |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Act 2, AT744.1, Ccl4, chemokine (C C motif) ligand 4, MIP 1B, Mip1b, Scya4 |
| Application: | CCL12, also known as MCP-5, is a small cytokine belonging to the CC chemokine family. It is a structural and functional homologue of human MCP-1. CCL12 is found predominantly in lymph nodes and thymus under normal conditions, and its expression can be markedly induced in activated macrophages both in vitro and in vivo. CCL12 is involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL4. The assay recognizes mouse CCL4. An antibody specific for mouse CCL4 has been pre-coated onto the microwells. The mouse CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CCL4 is added to detect the captured mouse CCL4 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .
Core Diagnostic Capabilities
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