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| Catalog: | C-EL-2111T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Mouse |
| Detection range: | 62.5-4000 pg/mL |
| Sensitivity: | 23.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | BAFF, BLyS, D8Ertd387e, TALL 1, TALL1, THANK, Tnfsf13b, TNFSF20, zTNF4 |
| Application: | Synaptic vesicle membrane docking and fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) complex which is located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). Other than VAMP2 and STX1A, SNAP25 is another key component of SNARE core complex, and is known to be involved in regulating neurotransmitter release. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues and mutation of the cysteines abolishes palmitoylation and its membrane binding activity. As an important presynaptic plasma membrane protein, SNAP25 has been linked to memory and learning by its effect on long term potentiation in the hippocampus, thus playing a critical role in the synaptic function of specific neuronal systems. It has been reported that SNAP25 levels were were significantly decreased in brain tissue homogenates of patients with later stages of Alzheimer's disease compared with the controls, while the levels of SNAP25 were significantly increased in cerebrospinal fluid (CSF) of the group with an Alzheimer's disease biomarker profile than in the group with a control biomarker profile. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BAFF ELISA kit is to be used to detect and quantify protein levels of endogenous BAFF. The assay recognizes mouse BAFF. An antibody specific for BAFF has been pre-coated onto the microwells. The BAFF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for BAFF is added to detect the captured BAFF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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