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Human VEGFR-1 ELISA Kit

  • Product Information
  • Description
Catalog: C-EL-2105T
Product Type: Test kit
Size: 96 tests
Principle: Sandwich ELISA
Species: Human
Detection range: 62.5-4000 pg/mL
Sensitivity: 7.8 pg/mL
Sample type: Serum, Plasma, Cell culture supernatants, Saliva
Test type: Quantitative
Analysis mode: ELISA
Storage: All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:: FLT, FLT 1, FLT1, Fms like tyrosine kinase 1, FRT, sflt1, Tyrosine protein kinase FRT, VEGFR 1, VEGFR1
Application: Uteroglobin, also named clara cell secretory protein or CC16, is mainly secreted by the club cells along the tracheobronchial tree, diffuses passively into the bloodstream and is rapidly eliminated by glomerular filtration. It is a steroid-dependent, immunomodulatory, cytokine-like protein, and it is secreted by the mucosal epithelial cells of many vertebrates and takes part in some signaling pathways. Growing evidence indicates that Uteroglobin has anti-inflammatory and anti-toxicant properties in the lung and may protect against obstructive lung diseases. Lower levels of Uteroglobin in blood and airways have been associated with prevalence and severity of Chronic obstructive pulmonary disease (COPD). Defects in Uteroglobin are associated with a susceptibility to asthma.

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGFR-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VEGFR-1. The assay recognizes human VEGFR-1. An antibody specific for VEGFR-1 has been pre-coated onto the microwells. The VEGFR-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGFR-1 is added to detect the captured VEGFR-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

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