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| Catalog: | C-EL-2103T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 125-8000 pg/mL |
| Sensitivity: | 1.7 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | CD106, DKFZp779G2333, INCAM 100, vascular cell adhesion molecule 1, vascular cell adhesion protein 1, VCAM 1, VCAM1 |
| Application: | Ubiquitin C-terminal hydrolase 1 (UCHL1) , previously known as PGP9.5, is found primarily in neurons and neuroendocrine cells with a very high concentration in dendrites and perikarya. UCHL1 is a more recently recognized biomarker of neuronal injury. UCHL1 could be detected in the CSF and serum with a half-life of 7-9 hours after severe traumatic brain injury. Increased plasma concentrations of UCHL1 therefore reflect increased blood brain barrier permeability and destruction of neurons. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VCAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VCAM-1. The assay recognizes human VCAM-1. An antibody specific for VCAM-1 has been pre-coated onto the microwells. The VCAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for VCAM-1 is added to detect the captured VCAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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