Human Total IL-18 ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL18 ELISA kit is to be used to detect and quantify protein levels of endogenous Pre-IL18 and mature IL18. The assay recognizes human IL18. An antibody specific for IL18 has been pre-coated onto the microwells. The IL18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL18 is added to detect the captured IL18 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.