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| Catalog: | C-EL-2088T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 62.5-4000 pg/mL |
| Sensitivity: | 6.9 pg/mL |
| Sample type: | Human serum,plasma, Cell culture supernatants, Saliva |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | CLGI, Collagenase inhibitor, EPA, EPO, FLJ90373, HCI, Metalloproteinase inhibitor 1, TIMP, TIMP 1, TIMP1 |
| Application: | Serotransferrin (TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TIMP1 ELISA kit is to be used to detect and quantify protein levels of endogenous TIMP1. The assay recognizes human TIMP1. An antibody specific for TIMP1 has been pre-coated onto the microwells. The TIMP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TIMP1 is added to detect the captured human TIMP1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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