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| Catalog: | C-EL-2085T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 1.56-100 ng/mL |
| Sensitivity: | 0.6 ng/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Urine |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin |
| Application: | Protein ST2, also known as IL1RL1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form (sST2), a transmembrane receptor form (ST2L), and a variant form of ST2 (ST2V). Interleukin-33 (IL-33) has been identified as a functional ligand of ST2L. IL-33 exerts its cellular functions by binding a receptor complex composed of ST2L and IL-1R accessory protein (IL-1RAcP). The interaction of IL-33 and ST2L activates mitogen-activated protein kinases and several biochemical pathways. The end of these reactions is the activation of the inhibitor of nuclear factor-kB (NF-kB) kinase complex, triggering NF-kB activity. sST2 seems to act as a decoy-receptor for IL-33: it binds IL-33 thus subtracting such a molecule from the interaction with ST2L. sST2 is formed by many cells and sST2 level is increased as response to myocardial stress, as well as in inflammatory conditions, including allergic asthma. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TF ELISA kit is to be used to detect and quantify protein levels of endogenous TF. The assay recognizes human TF. An antibody specific for TF has been pre-coated onto the microwells. The TF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TF is added to detect the captured TF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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