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| Catalog: | C-EL-2082T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 31.25-2000 pg/mL |
| Sensitivity: | 1.8 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1 |
| Application: | S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD). |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous ST2. The assay recognizes human ST2. An antibody specific for ST2 has been pre-coated onto the microwells. The ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ST2 is added to detect the captured ST2 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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