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| Catalog: | C-EL-2080T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 1.56 - 100 ng/mL , 6.25 - 400 ng/mL |
| Sensitivity: | 0.20 ng/mL |
| Sample type: | Serum, Plasma, Urine, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | A1A, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, alpha 1-Antitrypsin, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT |
| Application: | Resistin, a cysteine-rich hormone secreted primarily by rodent fat cells, was postulated to be implicated in obesity, insulin resistance and diabetes. Resistin is also secreted by macrophages in humans, promoting inflammation (PMID: 20148674). Resistin modulates the synthesis and secretion of key pro-inflammatory cytokines such as TNF-α, IL-6 and IL12 in macrophages via a nuclear factor-kB (NF-kB)-dependent pathway. Given the connection between inflammation and cancer, resistin was soon considered for its role in cancer. There were observations supporting its possible use as a diagnostic marker, expression levels of which also seemed to differ in patients with varying clinical stages (PMID: 30317640). Resistin has been also linked to other pathologies such as atherosclerosis (PMID: 15710760). |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA1. The assay recognizes human SERPINA1. An antibody specific for SERPINA1 has been pre-coated onto the microwells. The SERPINA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SERPINA1 is added to detect the captured SERPINA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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