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| Catalog: | C-EL-2066T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 125-8000 pg/mL |
| Sensitivity: | 43.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Cell lysates |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | CD279, hPD 1, hPD l, PD1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2 |
| Application: | CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. p21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. p21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. p21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PD1 ELISA kit is to be used to detect and quantify protein levels of endogenous PD1. The assay recognizes human PD1. An antibody specific for PD1 has been pre-coated onto the microwells. The PD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PD1 is added to detect the captured PD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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