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Human ORM1 ELISA Kit

  • Product Information
  • Description
Catalog: C-EL-2060T
Product Type: Test kit
Size: 96 tests
Principle: Sandwich ELISA
Species: Human
Detection range: 0.625-20 ng/mL
Sensitivity: 0.37 ng/mL
Sample type: Urine, Human serum
Test type: Quantitative
Analysis mode: ELISA
Storage: All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:: AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, orosomucoid 1
Application: Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Elevated serum levels of myoglobin are found in the bloodstream after muscle injury. Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction.

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ORM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ORM1. The assay recognizes human ORM1. An antibody specific for ORM1 has been pre-coated onto the microwells. The ORM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ORM1 is added to detect the captured ORM1 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

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