Human NGAL ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous NGAL. The assay recognizes human NGAL. An antibody specific for NGAL has been pre-coated onto the microwells. The NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for NGAL is added to detect the captured NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .