Human MPO ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MPO ELISA kit is to be used to detect and quantify protein levels of endogenous MPO. The assay recognizes Human MPO. An antibody specific for MPO has been pre-coated onto the microwells. The MPO protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MPO is added to detect the captured MPO protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.