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| Catalog: | C-EL-2106T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human/Mouse |
| Detection range: | 62.5-4000 pg/mL |
| Sensitivity: | 6.0 pg/mL |
| Sample type: | Cell lysates, Tissue lysates |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Component of gems 1, Gemin 1, SMN, SMN1, SMN2, SMNC, SMNT, Survival motor neuron protein |
| Application: | Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM-1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1. Increased sVCAM-1 has been shown to correlate with disease activity in various entities including rheumatoid arthritis, systemic lupus erythematosus, and lupus nephritis. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SMN ELISA kit is designed for the quantitative determination of SMN in samples of human and mouse origin. An antibody specific for SMN has been pre-coated onto the microwells. The SMN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SMN is added to detect the captured SMN protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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