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| Catalog: | C-EL-2045T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 15.6 - 500 pg/mL |
| Sensitivity: | 2.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | LEP, leptin, OB, Obese protein, Obesity factor, OBS |
| Application: | JNK is also named as MAPK8 (Mitogen-activated protein kinase 8), PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. JNK is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. JNK functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. JNK activity is abnormally elevated in obesity. Furthermore, an absence of JNK results in decreased adiposity, significantly improved insulin sensitivity, and enhanced insulin receptor signaling capacity in 2 different models of mouse obesity, including ob/ob. JNK is a crucial mediator of obesity and insulin resistance and a potential target for therapeutics. The ELISA kit is suitable for testing cell lysates. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human leptin ELISA kit is to be used to detect and quantify protein levels of endogenous human . The assay recognizes human leptin. An antibody specific for human leptin has been pre-coated onto the microwells. The human leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human leptin is added to detect the captured human leptin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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