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| Catalog: | C-EL-2038T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 3.8 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Urine |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | IL6 |
| Application: | Interleukin 34 (IL34) is a hematopoietic cytokine that acts as a key regulator of survival, proliferation and differentiation of myeloid lineage cells including monocytes, macrophages and osteoclasts. IL34 was identified in 2008 in a comprehensive proteomic analysis as a tissue-specific ligand of CSF-1 receptor (CSF-1R). This cytokine shares numerous common features with M-CSF, especially its receptor (MCSF-R) explaining partly their functional overlap. IL34 is expressed in various tissues and it is most abundant in the spleen. Upon inflammation, other cells, such as fibroblasts and articular synovial cells, upregulate IL34 expression. Recent studies have reported that IL34 levels are elevated in the serum, synovial fluid, and synovial tissue of RA patients, and like CSF-1, IL34 is induced by TNF and IL-1beta in RA fibroblast-like synoviocytes (FLS). |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL6 is added to detect the captured IL6 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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