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| Catalog: | C-EL-2030T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 7.8-500 pg/mL |
| Sensitivity: | 1.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Urine |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20 |
| Application: | Interleukin 22 (IL22) is a member of the human type I interferon family, which includes IL10. IL22 has the potential to interact with IL10 because it binds to the IL-10R2c chain with IL22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL22 binding protein (IL22BP). Although known as a Th17 cytokine, IL22 is also expressed by a wide range of immune cells, including NK T, γδ T, and NK cells. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL10. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL28A. The assay recognizes human IL28A. An antibody specific for IL28A has been pre-coated onto the microwells. The IL28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL28A is added to detect the captured IL28A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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