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| Catalog: | C-EL-2026T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 2.0 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | IL 21, IL21, IL-21, interleukin 21, Za11 |
| Application: | Interlukin-1 polypeptides are pleiotropic cytokines exerting diverse functions in different tissues. IL-1R1 (also known as CD121a) is a receptor for interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and interleukin-1 receptor antagonist (IL-1Ra). Human IL-1R1 was first cloned from human T cells. This receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be shed from the cell membrane as a soluble form (sIL-1R1) which can function as a soluble decoy receptor and prevent the binding of IL-1α, IL-1β, and IL-1Ra to membrane IL-1R1. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL21 ELISA kit is to be used to detect and quantify protein levels of endogenous IL21. The assay recognizes human IL21. An antibody specific for IL21 has been pre-coated onto the microwells. The IL21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL21 is added to detect the captured IL21 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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