Human IL-1R2 ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1R2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1R2. The assay recognizes human IL-1R2. An antibody specific for IL-1R2 has been pre-coated onto the microwells. The IL-1R2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL-1R2 is added to detect the captured human IL-1R2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.