USA: 
Europe: 
Email: 
Consultation
| Catalog: | C-EL-2008T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 0.156-10 ng/mL |
| Sensitivity: | 0.02 ng/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Urine |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | CD340, ERBB2, ERBB2,p185-Specific, HER 2, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1 |
| Application: | Gastrin-releasing peptide, also known as GRP, is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, and its main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Its 148-amino acid preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27-amino acid gastrin-releasing peptide or the 10-amino acid neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation.These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HER2 ELISA kit is to be used to detect and quantify protein levels of endogenous HER2. The assay recognizes human HER2. An antibody specific for HER2 has been pre-coated onto the microwells. The HER2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HER2 is added to detect the captured HER2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
Follow Us -
Copyright © 2024 Creative Biogene. All rights reserved.
