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| Catalog: | C-EL-2000T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 7.8-500 pg/mL |
| Sensitivity: | 0.6 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | 519, D2S69E, GNLY, granulysin, LAG 2, LAG2, Lymphokine LAG 2, NKG5, Protein NKG5, T cell activation protein 519, TLA519 |
| Application: | CSF2, also named as GM-CSF, is an important hematopoietic growth factor and immune modulator, which is produced by a variety of cell types including T cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. It was originally recognized as a stimulator for the proliferation of granulocytes and macrophages from bone marrow precursor cells. It has also been shown to promote the survival and activation of mature myeloid cells and therefore contributes to the maintenance of innate immune homeostasis. Recent studies suggest that GM-CSF also has proinflammatory functions and plays critical roles in the development of autoimmune and inflammatory diseases, particularly in Th17 driven diseases. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GNLY/Granulysin ELISA kit is to be used to detect and quantify protein levels of endogenous GNLY/Granulysin. The assay recognizes human GNLY/Granulysin. An antibody specific for GNLY/Granulysin has been pre-coated onto the microwells. The GNLY/Granulysin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GNLY/Granulysin is added to detect the captured GNLY/Granulysin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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