Human EGFR ELISA Kit

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The EGFR ELISA kit is to be used to detect and quantify protein levels of endogenous EGFR. The assay recognizes human EGFR. An antibody specific for EGFR has been pre-coated onto the microwells. The EGFR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for EGFR is added to detect the captured EGFR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .