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Human CX3CL1 ELISA Kit

  • Product Information
  • Description
Catalog: C-EL-1971T
Product Type: Test kit
Size: 96 tests
Principle: Sandwich ELISA
Species: Human
Detection range: 31.25-2000 pg/mL
Sensitivity: 1.9 pg/mL
Sample type: Human milk
Test type: Quantitative
Analysis mode: ELISA
Storage: All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:: ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1
Application: CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth (PMID: 12826659). It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 is a HIF-1α regulated, secreted angiogenic growth factor that promotes smooth muscle cells migration, proliferation and tissue revascularization (PMID: 25589425). CNPY2 enhances cell spreading in fibroblasts and migration of rat C6 glioma cells (PMID: 15905959). CNPY2 was also shown to influence the expression of low-density lipoprotein receptor and FGF21 (PMID: 22378787). This kit is used to quantify CNPY2 level in vivo.

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CX3CL1 ELISA kit is to be used to detect and quantify protein levels of endogenous CX3CL1. The assay recognizes human CX3CL1. An antibody specific for CX3CL1 has been pre-coated onto the microwells. The CX3CL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for CX3CL1 is added to detect the captured CX3CL1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .

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