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| Catalog: | C-EL-1970T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 0.156-10 ng/mL |
| Sensitivity: | 0.02 ng/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | C reactive protein, CRP, PTX1 |
| Application: | Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement. Increased plasma clusterin levels have been associated with severity, pathology, and progression in Alzheimer disease. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CRP ELISA kit is to be used to detect and quantify protein levels of endogenous CRP. The assay recognizes human CRP. An antibody specific for CRP has been pre-coated onto the microwells. The CRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CRP is added to detect the captured CRP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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