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| Catalog: | C-EL-1965T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 31.25-2000 pg/mL |
| Sensitivity: | 2.2 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants, Urine |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor |
| Application: | CD147, also called Basigin (BSG) or EMMPRIN, is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily and is important in numerous physiological and pathological phenomena. It is involved in reproduction, neural function, inflammation and tumor invasion. CD147 is broadly distributed, and expressed at particularly high levels at the retinal pigment epithelium and neonatal blood-brain barrier, on tumour cells and on activated T cells. CD147 can stimulate adjacent fibroblasts to produce matrix metalloproteinases (MMPS). It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers. Soluble CD147 (sCD147) has been uncovered in addition to membrane-bound CD147. sCD147 production is mediated by two different mechanisms: microvesicular secretion and proteolytic cleavage. The presence of sCD147 in body fluids is associated with several diseases such as systemic sclerosis, hepatocellular carcinoma, ankylosing spondylitis and coronary diseases. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD23 ELISA kit is to be used to detect and quantify protein levels of endogenous CD23. The assay recognizes human CD23. An antibody specific for CD23 has been pre-coated onto the microwells. The CD23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CD23 is added to detect the captured CD23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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