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| Catalog: | C-EL-1958T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 15.6-1000 pg/mL |
| Sensitivity: | 3.4 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | ACT 2, ACT2, CCL4, MIP 1 beta |
| Application: | CCL24, known as eotaxin-2, is a chemokine that promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the C-C motif chemokine receptor 3 (CCR3) complex. It is expressed by activated T cells, monocytes, epithelial and endothelial cells as well as by activated fibroblasts. CCL24 is considered a contributor to kinds of atopic disorders, parasitic infections and numerous systemic diseases. CCL24 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL4. The assay recognizes human CCL4. An antibody specific for CCL4 has been pre-coated onto the microwells. The CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL4 is added to detect the captured human CCL4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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