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| Catalog: | C-EL-1954T |
| Product Type: | Test kit |
| Size: | 96 tests |
| Principle: | Sandwich ELISA |
| Species: | Human |
| Detection range: | 7.8-500 pg/mL |
| Sensitivity: | 0.6 pg/mL |
| Sample type: | Serum, Plasma, Cell culture supernatants |
| Test type: | Quantitative |
| Analysis mode: | ELISA |
| Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms:: | A 152E5.1, C C motif chemokine 22, CC chemokine STCP 1, CCL22, DC/B CK, Macrophage derived chemokine, MDC, MDC(1 69), SCYA22, Small inducible cytokine A22, STCP 1 |
| Application: | BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 is a glycosylated disulfide-linked 36 kDa homodimeric protein, having role in the induction, development, and regulation of adipocytes, especially brown adipose tissue (BAT). BMP7 is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation. Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival. Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL22/MDC ELISA kit is to be used to detect and quantify protein levels of endogenous CCL22/MDC. The assay recognizes human CCL22/MDC. An antibody specific for CCL22/MDC has been pre-coated onto the microwells. The CCL22/MDC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL22/MDC is added to detect the captured human CCL22/MDC protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Core Diagnostic Capabilities
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