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Human BAFF ELISA Kit

  • Product Information
  • Description
Catalog: C-EL-1950T
Product Type: Test kit
Size: 96 tests
Principle: Sandwich ELISA
Species: Human
Detection range: 7.8-500 pg/mL
Sensitivity: 0.5 pg/mL
Sample type: Serum, Plasma, Cell Culture supernatants
Test type: Quantitative
Analysis mode: ELISA
Storage: All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:: B cell activating factor, B lymphocyte stimulator, BAFF, BLYS, CD257, DTL, TALL 1, TALL1, THANK, TNFSF13B, TNFSF20, ZTNF4
Application: alpha-synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. a-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV).

This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BAFF ELISA kit is to be used to detect and quantify protein levels of endogenous BAFF. The assay recognizes human BAFF. An antibody specific for BAFF has been pre-coated onto the microwells. The BAFF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BAFF is added to detect the captured human BAFF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

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