Catalog: | C-EL-1940T |
Product Type: | Test kit |
Size: | 96 tests |
Principle: | Sandwich ELISA |
Species: | Human |
Detection range: | 62.5 - 4000 pg/mL |
Sensitivity: | 17.3 pg/mL |
Sample type: | Serum, Plasma, Saliva |
Test type: | Quantitative |
Analysis mode: | ELISA |
Storage: | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
Synonyms:: | AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003 |
Application: | AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma levels decrease rapidly after birth. AFP is one of the earliest markers of the hepatocyte lineage. High AFP concentrations have been correlated with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. |
This test kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 1. The assay recognizes human Angiopoietin 1. An antibody specific for Angiopoietin 1 has been pre-coated onto the microwells. The Angiopoietin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Angiopoietin 1 is added to detect the captured human Angiopoietin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
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