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Flow Cytometry

The Principle of Flow CytometryFlow cytometry (FCM) is a technology developed in the 1970s to quickly and accurately identify and classify immune cells and other cells. It mainly includes fluid flow technology, cell sorting and counting technology, as well as data collection and analysis technology, etc. At present, FCM has become an important biological tool for detecting various components of cells. It can not only quantitatively detect various cellular components in cell membranes, cytoplasm, nucleus, and various soluble biomolecular components in serum, but also study various functional states of cells. In recent decades, with the continuous discovery of new fluorescent substances and the increasing improvement of computer functions in data storage, display analysis, etc., FCM has transitioned from the previous qualitative description to quantitative research, and can conduct data statistics, which has greatly improved the scientific nature of experimental results. Based on the above characteristics, FCM has become an important research technique for clinical diagnosis and has a wide application prospect.

The Workflow of Flow Cytometry

The workflow of flow cytometry is as follows. Planning the workflow in advance as shown in the figure helps to improve the success rate of the experiment.

The Workflow of Flow Cytometry – Creative Biogene

Applications of Flow Cytometry

Flow cytometry is widely used in basic research and clinical practice, and plays an important role in the fields of cell biology, hematology, immunology, oncology, pharmacology, genetics, and clinical testing. It can assist in the diagnosis of various diseases such as blood diseases, cancer, autoimmune diseases, antiviral infections and so on. One of the most common applications is the diagnosis of blood-related cancers, such as leukemia and lymphoma. Take leukemia as an example, use fluorescent dye-treated specific monoclonal antibodies, such as CD2, CD7, CD10, CD14, CD19, CD33, CD34, CD41, CD64 and HLA-DR, etc., to detect the presence or absence of various cell components commonly seen in leukemia, thus achieving the purpose of diagnosis. In the early stage of malignant disease, there may be only a few cancer cells in the sample, and routine examination under the microscope may not be able to find these cancer cells, so flow cytometry is particularly useful in the early stage of malignant disease.

Advantages of Flow Cytometry

Flow cytometry is a new technology that can quickly, accurately, and high-throughput the characteristics or functions of a single particle to perform multi-parameter qualitative and quantitative analysis. The advantage of FCM is that it can quickly measure multiple indicators at the same time, and it transitions the diagnosis result from the previous qualitative description to the quantitative research, which greatly improves the scientific nature of the results.

Creative Biogene has accumulated years of experience in flow cytometry. We promise to provide you with the best diagnostic products. You can choose us with confidence.

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Reference

  1. Peters J M., and Ansari M Q. (2011). "Multiparameter flow cytometry in the diagnosis and management of acute leukemia." Archives of pathology & laboratory medicine. 135.1: 44-54.
All Products
Cat# Product Name Product Type Inquiry
C-FC-0661A APC Anti-Mouse CD183/CXCR3 Antibody FCM Antibody Inquiry
C-FC-0662A APC Anti-Mouse CD19 Antibody FCM Antibody Inquiry
C-FC-0663A APC Anti-Mouse CD19 Antibody FCM Antibody Inquiry
C-FC-0664A APC Anti-Mouse CD1d Antibody FCM Antibody Inquiry
C-FC-0665A APC Anti-Mouse CD1d Antibody FCM Antibody Inquiry
C-FC-0666A APC Anti-Mouse CD200 Antibody FCM Antibody Inquiry
C-FC-0667A APC Anti-Mouse CD200 Antibody FCM Antibody Inquiry
C-FC-0668A APC Anti-Mouse CD206 Antibody FCM Antibody Inquiry
C-FC-0669A APC Anti-Mouse CD206 Antibody FCM Antibody Inquiry
C-FC-0670A APC Anti-Mouse CD22 Antibody FCM Antibody Inquiry
C-FC-0671A APC Anti-Mouse CD22 Antibody FCM Antibody Inquiry
C-FC-0672A APC Anti-Mouse CD23 Antibody FCM Antibody Inquiry
C-FC-0673A APC Anti-Mouse CD23 Antibody FCM Antibody Inquiry
C-FC-0674A APC Anti-Mouse CD24 Antibody FCM Antibody Inquiry
C-FC-0675A APC Anti-Mouse CD24 Antibody FCM Antibody Inquiry
C-FC-0676A APC Anti-Mouse CD25 Antibody FCM Antibody Inquiry
C-FC-0677A APC Anti-Mouse CD25 Antibody FCM Antibody Inquiry
C-FC-0678A APC Anti-Mouse CD272 Antibody FCM Antibody Inquiry
C-FC-0679A APC Anti-Mouse CD272 Antibody FCM Antibody Inquiry
C-FC-0680A APC Anti-Mouse CD274/PD-L1 Antibody FCM Antibody Inquiry
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