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Flow Cytometry

The Principle of Flow CytometryFlow cytometry (FCM) is a technology developed in the 1970s to quickly and accurately identify and classify immune cells and other cells. It mainly includes fluid flow technology, cell sorting and counting technology, as well as data collection and analysis technology, etc. At present, FCM has become an important biological tool for detecting various components of cells. It can not only quantitatively detect various cellular components in cell membranes, cytoplasm, nucleus, and various soluble biomolecular components in serum, but also study various functional states of cells. In recent decades, with the continuous discovery of new fluorescent substances and the increasing improvement of computer functions in data storage, display analysis, etc., FCM has transitioned from the previous qualitative description to quantitative research, and can conduct data statistics, which has greatly improved the scientific nature of experimental results. Based on the above characteristics, FCM has become an important research technique for clinical diagnosis and has a wide application prospect.

The Workflow of Flow Cytometry

The workflow of flow cytometry is as follows. Planning the workflow in advance as shown in the figure helps to improve the success rate of the experiment.

The Workflow of Flow Cytometry – Creative Biogene

Applications of Flow Cytometry

Flow cytometry is widely used in basic research and clinical practice, and plays an important role in the fields of cell biology, hematology, immunology, oncology, pharmacology, genetics, and clinical testing. It can assist in the diagnosis of various diseases such as blood diseases, cancer, autoimmune diseases, antiviral infections and so on. One of the most common applications is the diagnosis of blood-related cancers, such as leukemia and lymphoma. Take leukemia as an example, use fluorescent dye-treated specific monoclonal antibodies, such as CD2, CD7, CD10, CD14, CD19, CD33, CD34, CD41, CD64 and HLA-DR, etc., to detect the presence or absence of various cell components commonly seen in leukemia, thus achieving the purpose of diagnosis. In the early stage of malignant disease, there may be only a few cancer cells in the sample, and routine examination under the microscope may not be able to find these cancer cells, so flow cytometry is particularly useful in the early stage of malignant disease.

Advantages of Flow Cytometry

Flow cytometry is a new technology that can quickly, accurately, and high-throughput the characteristics or functions of a single particle to perform multi-parameter qualitative and quantitative analysis. The advantage of FCM is that it can quickly measure multiple indicators at the same time, and it transitions the diagnosis result from the previous qualitative description to the quantitative research, which greatly improves the scientific nature of the results.

Creative Biogene has accumulated years of experience in flow cytometry. We promise to provide you with the best diagnostic products. You can choose us with confidence.

Please contact us for more details.

Reference

  1. Peters J M., and Ansari M Q. (2011). "Multiparameter flow cytometry in the diagnosis and management of acute leukemia." Archives of pathology & laboratory medicine. 135.1: 44-54.
All Products
Cat# Product Name Product Type Inquiry
C-FC-2961A Anti-CD23 Antibody FCM Antibody Inquiry
C-FC-2962A Anti-CD25/IL2RA Antibody (APC) FCM Antibody Inquiry
C-FC-2963A Anti-CD25/IL2RA Antibody (FITC) FCM Antibody Inquiry
C-FC-2964A Anti-CD25/IL2RA Antibody (PE) FCM Antibody Inquiry
C-FC-2965A Anti-CD25/IL2RA Antibody (PerCP) FCM Antibody Inquiry
C-FC-2966A Anti-CD25/IL2RA Antibody FCM Antibody Inquiry
C-FC-2967A Anti-CD27 Antibody (APC) FCM Antibody Inquiry
C-FC-2968A Anti-CD27 Antibody (FITC) FCM Antibody Inquiry
C-FC-2969A Anti-CD27 Antibody (PE) FCM Antibody Inquiry
C-FC-2970A Anti-CD27 Antibody (PerCP) FCM Antibody Inquiry
C-FC-2971A Anti-CD27 Antibody FCM Antibody Inquiry
C-FC-2972A Anti-CD28 Antibody FCM Antibody Inquiry
C-FC-2973A Anti-CD3 epsilon/CD3e Antibody (APC) FCM Antibody Inquiry
C-FC-2974A Anti-CD3 epsilon/CD3e Antibody (FITC) FCM Antibody Inquiry
C-FC-2975A Anti-CD3 epsilon/CD3e Antibody (PE) FCM Antibody Inquiry
C-FC-2976A Anti-CD3 epsilon/CD3e Antibody (PerCP) FCM Antibody Inquiry
C-FC-2977A Anti-CD3 epsilon/CD3e Antibody FCM Antibody Inquiry
C-FC-2978A Anti-CD30/TNFRSF8 Antibody (APC) FCM Antibody Inquiry
C-FC-2979A Anti-CD30/TNFRSF8 Antibody (FITC) FCM Antibody Inquiry
C-FC-2980A Anti-CD30/TNFRSF8 Antibody (PE) FCM Antibody Inquiry
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