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| Catalog: | C-FC-4759A |
| Product Type: | FCM Antibody |
| Size: | 0.5 mg/ml |
| Reactivity: | Mouse |
| Positive FC: | mouse bone marrow cells |
| Recommended dilution: | This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. |
| Analysis mode: | FC |
| Host: | Rat |
| Isotype: | IgG2a |
| Alternate names: | Gr 1, Gr1, Ly 6G, Ly6g |
| Storage: | 2-8°C |
| Purification method: | Affinity purification |
| Immunogen: | Ly-6G transfected EL-4J cell line |
| Genn_ID: | 546644 |
| Gene accession: | X70920 |
| Gene symbol: | Ly6g |
| Buffer: | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).
Core Diagnostic Capabilities
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